EK-hemocyte
Order
Species
Common Name(s)
Mediterranean flour moth
Other Designations
EK-hemocyte cell line
Stage
Tissue
Initiator(s)
Bita Valizadeh
References
Valizadeh, B., Samarfard, S., Sendi, J. J., & Karbanowicz, T. P. (2020). Developing an Ephestia kuehniella hemocyte cell line to assess the bio-insecticidal potential of microencapsulated Helicoverpa armigera nucleopolyhedrovirus against cotton bollworm (Lepidoptera: Noctuidae) Larva. Journal of Economic Entomology, 113(5), 2086-2095.
Notes
Hemocyte-derived cell line with multiple hemocyte morphotypes: prohemocytes, plasmatocytes, granulocytes, oenocytoids, spherulocytes, vermicytes, podocytes; Population doubling time: 2.3 days in EX-CELL 420, 3.7 days in Grace’s medium; Highly susceptible to HearNPV infection (up to 80% polyhedra-forming in Ex-Cell); Used to test microencapsulated HearNPV formulations A and B; Suitable for virus production, metabolic studies, and baculovirus bio-insecticide screening.
Medium
EX-CELL® 420 + 20–10% FBS; Grace’s medium + 20–10% FBS; (Cultured at 28 °C)
Cell Culture Collection
University of Guilan, Iran; University of Queensland
jjalali@guilan.ac.ir
Status
Microorganism Propagation
Efficient propagation of HearNPV (MOI 1–2 PFU/cell).